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HPLC and LCMS Columns
Echelon HPLC Columns Lancer C8, C18, Cyano & Phenyl HPLC Columns Highload C18 HPLC Columns Advantage 100 C8 and C18 HPLC Columns
Advantage 300+ C4 and C18 HPLC Columns Fleet Direct Connect HPLC Columns Sprite Direct Connect HPLC Columns FluroPhase HPLC Columns
 Go Back to Column Comparison Guide Guard Columns and Filters

Advantage™ Ion Exchange Columns

Advantage™ Anion Exchange Columns

High resolution and loading capacity
Excellent recovery of biomolecules


Weak Anion Exchange HPLC Columns
6, 300Á, Spherical Silica


This weak anion exchanger is a crosslinked polyethyleneimine support. It is compatible with aqueous and many organic solvents in the pH range of 2-8. Selectivity can be altered by mobile phase composition and pH affects ionization of both the support and the solute. Our anion exchange columns permit analysis of proteins up to molecular weights of 200,000 daltons and have excellent recoveries and loading capacity. For example, 22mg ovalbumin can be loaded onto a 250 x 4.6 column with no overloading effects. Chromatofucusing can also be performed on our weak anion exchange columns due to their broad titration curves and high buffering capacity.

Cat. No. Description
ADV2110AX5 100mm x 2.1mm Column
ADV2115AX5 150mm x 2.1mm Column
ADV2125AX5 250mm x 2.1mm Column
ADV4610AX5 100mm x 4.6mm Column
ADV4615AX5 150mm x 4.6mm Column
ADVAX5G 10mm x 3.2mm Weak Anion Exhange Guard Cartridges, 3/Pkg
ADVGPC-GH Ion Exchange Guard Holder
ADVAX300 AX300, 10g Bottle




Strong Anion Exchange HPLC Columns
6, 300, Spherical Silica


The strong anion exchanger is quaternized, crosslinked polyethyleneimine support. It is compatible with aqueous and many organic solvents in the pH range of 2-8. Ionization of our strong anion exchange columns have no pH dependence. This support is an excellent column for the rapid separation of proteins and enzymes.

Cat. No. Description
ADV2110AX7 100mm x 2.1mm Column
ADV2125AX7 250mm x 2.1mm Column
ADV4610AX7 100mm x 4.6mm Column
ADV4615AX7 150mm x 4.6mm Column
ADVAX7G 10mm x 3.2mm Strong Anion
ADVGPC-GH Ion Exchange Guard Holder
ADVQ300 Q300, 10g Bottle



CM (WCX) Specifications for Solute Interaction
Pore
Size
Optimum
Molecular Wt
Surface
Area
Loading
Capacity
Ion Exchange
Capacity
300Á <200,000 100m2/g 80mg/mL 30mg hemoglobin
per gram support
Advantage™ Cation Exchange Columns

High resolution and loading capacity
Excellent recovery of biomolecules


Weak Cation Exchange HPLC Columns
6, 300Á, Spherical Silica


This weak cation exchanger has a polyamide coating containing carboxymethyl groups. It is compatible with aqueous and many organic solvents in the pH range of 2-8. Selectivity can be altered by mobile phase salt composition and pH affects ionization of both the support and the solute. Our cation exchange columns permit analysis of proteins up to molecular weights of 200,000 daltons and have excellent recoveries and loading capacity.

Cat. No. Description
ADV2110CX5 100mm x 2.1mm Column
ADV2125CX5 250mm x 2.1mm Column
ADV4610CX5 100mm x 4.6mm Column
ADV4625CX5 250mm x 4.6mm Column
ADVCX5G 10mm x 3.2mm Weak Cation, Exch. Guard Cartridges, 3/Pkg
ADVGPC-GH Advantage™ Ion Exchange Guard Holder



Strong Cation Exchange HPLC Columns
6, 300, Spherical Silica


The strong cation exchanger has a polyamide coating containing sulfopropyl functional groups. It is compatible with aqueous and many organic solvents in the pH range of 2-8. Ionization of the functional groups on this bonded phase is constant above pH 3. Our cation exchange columns are a great choice for separations of basic proteins.

Cat. No. Description
ADV2110CX7 100mm x 2.1mm Column
ADV2125CX7 250mm x 2.1mm Column
ADV4610CX7 100mm x 4.6mm Column
ADV4625CX7 250mm x 4.6mm Column
ADVCX7G 10mm x 3.2mm Strong Cation Exchange Guard Cartridges, 3/Pkg
ADVGPC-GH Ion Exchange Guard Holder



CM (WCX) Specifications for Solute Interaction
Pore
Size
Optimum
Molecular Wt
Surface
Area
Loading
Capacity
Ion Exchange
Capacity
300Á? <200,000 100m2/g 80mg/mL 65mg
hemoglobin
per gram
support


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